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Anti Inflamatory Activity

        Phytochemical Screening of Ficus glomerata. Roxb. Galled leaves
               
- by A. Sudhakar*¹, M. Asrar Sheriff², A. K. Sultan Mohideen², Shaik Azeem Taj 3
 

Abstract

Ficus glomerata. Roxb. commonly called as “Fig tree” is traditionally popular for its medicinal properties. The psyllid insect Pauropsylla depressa was identified in the present study to induce leaf galls in F. glomerata. Preliminary phytochemical screening of the various extracts of galled leaves in F.glomerata showed the presence of alkaloids, steroids, quinones and phenols in addition to fluorescence compounds. The therapeutic properties of these phytochemicals and their pharmacological significance are outlined.

Keywords: Ficus glomerata, phytochemical studies, galled leaves, Psyllid insect.
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Introduction
India has a rich source of plant biodiversity possessing tremendous medicinal properties. Ficus glomerata. Roxb. is a popular medicinal plant in India, which has long been used in Ayurveda for the treatment of diabetes, liver disorders, respiratory, urinary diseases and inflammatory conditions[¹]. Commonly called as the “fig tree”. F. glomerata extracts have also been reported to possess significant medicinal and pharmacological properties like anti-microbial, anti-cancer and anti-oxidant activity [2].Many parts of the fig plant like the trunk, leaves, flowers etc. are attacked by a number of pathogens including fungi, bacteria and insects. Leaf galls are abnormal outgrowths commonly seen in F. glomerata, produced in response to the feeding or oviposition stimulus of insects resulting in excessive growth and cell multiplication [3] Considering their wide distribution in India and varied medicinal uses, the normal and galled leaves of F. glomerata were subjected to phytochemical screening to qualitatively determine the occurrence of various constituents like phenols, steroids, and alkaloids etc.,.

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Materials and Methods
Ficus glomerata leaves were collected from Perambur, Chennai district, Tamilnadu. Normal and galled leaves were washed, cleaned and dried for further use. Leaf extracts were prepared following the cold filtration method[4] with 50% ethanol and distilled water as the solvents. The extractive value was calculated. Qualitative phytochemical analysis of the normal and galled leaf extracts was done following the procedures of Trease & Evans (1986)[5] for alkaloids (Dragendroff’s reagent), terpenoids(Noller’s test), tannins(1% lead acetate), saponins(Foam test), flavanoids(Shinoda test), phenols(Ferric chloride test), steroids(Libermann Burchard test). Fluorescence studies of dried powders of normal and galled leaves with various reagents like acetic acid, concentrated hydrochloric acid and sodium hydroxide was determined under daylight and UV light following the method of Trease & Evans(1986)[5]
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Results

The normal leaves of F. glomerata were dark green in colour with no bulbous outgrowths or masses. The galled leaves were globular, yellowish or reddish brown in colour, devoid of chlorophyll. The galls measured about 15-30mm in diameter. The insect Pauropsylla depressa (adult and a few larval stages) was collected from the galled leaves and identified (Fig 1). They belong to the order hemiptera and family psylloidea. The extractive values of F. glomerata leaves was 14% w/v in water and 15% w/v with ethanol as solvent. Preliminary phytochemical screening of F. glomerata leaves showed the presence of

saponins, alkaloids, steroids, tannins and phenols in both normal and galled leaves and the presence of coumarins and quinones only in the galled leaves(Table 1). Fluorescence studies of normal leaf powders showed the presence of black fluorescence with acetic acid and Conc.HCl while the galled leaf powders showed brown fluorescence in acetic acid and green fluorescence with NaOH and conc. HCl under UV light(Table 2).

Fig 1: Adult and Larval Stage of Pauropsylla depressa Adult (10X)

Larva(40X)

Table 1: Phytochemical Screening of Ficus glomerata extracts

+ = Presence; - = absence; NA = Normal aqueous extract; GA = Galled aqueous extract; NE = Normal ethanol extract; GE = Galled ethanol extract.

Table 2: Fluorescence analysis of Ficus glomerata leaf extracts

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Discussion
Phytochemicals are naturally occurring; biologically active, non-nutritive chemical compounds found in plants and act as a natural defense system against various pests. Various phytochemicals have been known to possess medicinal properties and hence widely used in Indian systems of traditional medicine[6]. In this study, various phytochemicals like saponins, alkaloids, tannins, etc were present in both normal and galled leaves of F. glomerata indicating their potential medicinal uses. Flavanoids tannins and alkaloids have anti-diabetic and anti-inflammatory activity [7,8]. Steroids and saponins have analgesic properties and central nervous system activities [9]. The galled leaves also showed higher contents of phytochemicals which is in accordance with the reports of Akkad [10] and Motta et.al [11] who reported variations in sugars and phtohormones in gall tissue when compared to normal plant tissue.
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Conclusion
Phytochemical screening of normal and galled leaves of F. glomerata revealed the occurrence of a variety of pharmacologically significant compounds like flavanoids, tannins, alkaloids, steroids and saponins which are known for their medicinal and therapeutic properties. Fluorescence studies of powder with various reagents revealed the presence of green fluorescence with conc. HCl and NaOH under UV light for galled leaves. The present preliminary study could be used as a tool for further detailed analysis to standardize the crude drugs and to conduct clinical trials to investigate the unexploited potential of this plant in the pharmaceutical/herbal industry.
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References
1. Ahmed, F and Urooj, A. Traditional uses, medicinal properties and phyto-pharmacology of Ficus racemosa : a review , Pharm. Biol., 48(6):672-681(2010).

2. Joseph, B. & Raj, S.J. Phyto-pharmacological and phytochemical properties of three Ficus species– an overview. Int.J. Pharma & BioSci., 1(4): 246-253(2010).

3. Ananthakrishnan, T. N. General and Applied Entomology, Tata-McGraw Hill Publ. 1184p (2004).

4. Dhanaraj, T.S., Murugaiah, K. and Jegadeesan, M. Phytochemical andalysis and antimicrobial activity of Aegle marmelos (L.)Corr. Herbal Tech Ind. 8(4): 7-10(2011).

5. Trease, G.E. & Evans, W.C. Pharmacognosy,(12th edition) Bailliere Tindall Eastbourne, 74-123(1983)

6. Upadhye, A.S. & Rajopadhye, A.A. Pharmacognosic and phytochemical evaluation of leaf galls of Kakad shringi used in Indian system of medicine. J.Sci.Ind.Res. 69: 700-704(2010). edition) Bailliere Tindall Eastbourne, 74-123(1983).

7. Augusti, K.T. and Cherian, S. Insulin sparing action of leucopelar gonidin derivative isolated from Ficus bengalensis Linn. Indian. J. Exp.Biol., 33:608-611(2008).

8. Paanakh, P & Ravichandra, V.D. Pharmacognostic and phytochemical investigation on leaves of Ficus hispida. Int. J. Pharm.PharmSci. 3(2) : 131-134(2011).

9. Mandal, S.C., Maity, T.K., Das, J., Saba, B.P and Pal, M. Anti-inflammatory evaluation of Ficus racemosa Linn. Leaves extract. J. Ethnopharmacol. 72:87-92(2009).

10. Akkad, S.E. Biochemical changes induced in Populus nigra leaves by galling aphids pemphigous populi. Int. J. Agri & Biol. 75: 672-686(2004).

11. Motta, B. Distribution of metabolites in galled and non-galled foliar tissues of Tibouchina pulchra. Biochem. Sys & Ecol. 33(10) : 971-981(2004).

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